Detecting COVID-19 Using Reverse-Transcription PCR (RT-PCR)

Edvotek Kit: Detecting COVID-19 Using Reverse-Transcription PCR (RT-PCR)

Reverse-transcription PCR (RT-PCR) is the gold standard for the detection of SARS-CoV-2 due to the sensitivity, specificity, and feasibility of the test. In this simulated RT-PCR experiment, students will explore the diagnostic test used worldwide to diagnose and monitor the spread of COVID-19. This experiment requires a PCR thermal cycler.

Please note, this experiment is a DNA simulation of reverse transcription PCR and is intended solely for educational purposes. There are no viral or human DNA products included in this experiment. The experiment will not perform viral RNA testing for SARS-CoV-2.

Kit Includes:

• Instructions
• PCR EdvoBeads™, LyphoPrimer™ Mix
• EdvoQuick™ DNA Ladder, DNA Templates
• TE Buffer, UltraSpec-Agarose™, TBE Electrophoresis Buffer powder
• SYBR® Safe Stain, FlashBlue™ DNA Stain
• Microcentrifuge & PCR Tubes..

You Need:

• DNA Electrophoresis
• Thermal cycler
• Micropipettes 5-50ul
• UV or Blue light transilluminator, white light box if staining with FlashBlue™
• Microcentrifuge, microwave or hotplate

Storage:

Some Components Require Freezer Storage Upon Receipt

Group Size: For 25 students working in 5 groups

Time Required:

• Set up - 30 minutes 
• PCR - 2 hours or overnight 
• Electrophoresis - 45 minutes