In Search of the Sickle Cell Gene by Southern Blot

Edvotek Kit: In Search of the Sickle Cell by Southern Blot

Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.

Kit Includes:

• Instructions.
• Ready-to-Load™ DNA samples.
• Agarose.
• Electrophoresis buffer.
• Nylon membranes.
• Filter paper.
• Blot stain.

Not Included:

• Electrophoresis apparatus.
• Power supply.
• Microwave or hot plate.
• Water bath.
• 80ºC incubation oven.

Some components require freezer storage.

Group Size: For 5 lab groups

Time Required:

• Electrophoresis - 45 minutes
• Blot - overnight
• Stain & Destain - 10 minutes