Using CRISPR To Treat Cystic Fibrosis

Edvotek Kit: Using CRISPR To Treat Cystic Fibrosis

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis.

Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

Kit Includes:

• Instructions.
• Ready-to-Load QuickStrip™ DNA Samples.
• UltraSpec-Agarose™.
• Electrophoresis Buffer (50X).
• 10X Gel Loading Solution.
• FlashBlue™ DNA Stain.

You Need:

• DNA Electrophoresis.
• Micropipettes: 5-50 µl (Optional).
• White Light Box.
• Microwave or Hot Plate.

Storage:

Room temperature stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.

Group Size: For 8 Gels

Time Required:

• Complete in 45 minutes