Sickle Cell Anaemia is a common genetic disease that causes long rods in red blood cells, giving them a 'sickled' appearance. These cells get stuck in small capillaries of the blood stream leading to oxygen deprivation that causes pain and organ damage. Sickle Cell Anemia is caused by a single point mutation in the hemoglobin gene that results in a faulty protein. In this experiment, your students will investigate the restriction enzyme that discriminates between HbA (normal) and HbS (disease) genes and perform a simulated test on a patient.
Kit includes: instructions, Ready-to-Load™ DNA samples, UltraSpec-Agarose™ powder, practice gel loading solution, electrophoresis buffer, InstaStain® Blue and FlashBlue™ stain,, calibrated pipette, microtipped transfer pipettes.
All you need: electrophoresis tank, power supply, variable micropipette and tips, balance and microwave or hot plate.
For 8 Gels. Complete in 45 minutes
Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.